Part:BBa_J100268

pJC184d5

In their 2014 paper "[http://www.nature.com/nchembio/journal/v10/n3/full/nchembio.1453.html Negative selection and stringency modulation in phage-assisted continuous evolution]," Carlson et al., designed part J100268 to be used as an accessory plasmid in phage-assisted continuous evolution (PACE). This DNA part comes from a collection of 8 plasmids numbers J100264 - J100271. PACE employs M13 phage and E. coli in a chemostat.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 2877
  Illegal EcoRI site found at 5360
  Illegal XbaI site found at 7731
  Illegal PstI site found at 4814
  Illegal PstI site found at 6294
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 2877
  Illegal EcoRI site found at 5360
  Illegal PstI site found at 4814
  Illegal PstI site found at 6294
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 2877
  Illegal EcoRI site found at 5360
  Illegal BglII site found at 3415
  Illegal BglII site found at 6176
  Illegal BamHI site found at 2024
  Illegal BamHI site found at 2293
  Illegal BamHI site found at 4434
  Illegal BamHI site found at 6963
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 2877
  Illegal EcoRI site found at 5360
  Illegal XbaI site found at 7731
  Illegal PstI site found at 4814
  Illegal PstI site found at 6294
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 2877
  Illegal EcoRI site found at 5360
  Illegal XbaI site found at 7731
  Illegal PstI site found at 4814
  Illegal PstI site found at 6294
  Illegal AgeI site found at 1859
  Illegal AgeI site found at 2165
  Illegal AgeI site found at 2654
  Illegal AgeI site found at 2852
  Illegal AgeI site found at 3313
  Illegal AgeI site found at 3536
  Illegal AgeI site found at 4709
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 5828
  Illegal SapI site found at 1841
  Illegal SapI.rc site found at 3503